New ITS applications for metropolitan areas based on Floating Car Data

The paper describes a couple of FCD based vehicular traffic applications and services. This new method is especially beneficial for regions with a poor traffic monitoring infrastructure because the necessary monetary effort to establish such a system is very small in comparison to conventional systems and it is flexible and easily adaptable to other regions. Particularly, emerging markets like China with a fast-changing road network and a high penetration of lat-est information technologies on one side but with serious foreseeable traffic related problems on the other side can surely profit from this approach. The new data collection and analysing methods result in better performance of the services enhance the scope of the services and hopefully enlarge user acceptance. All of the proposed solutions are prototypes and not all of them have been extensively tested up to now. Certainly, specific data processing methods need further research, some refinements and calibrations. Additionally, some applications still suffer from insufficient data penetration. Nevertheless, the approach is very general and it is very likely that FCD availability will sharply increase in near future and will enhance the quality of services

Monitoring Traffic and Emissions by Floating Car Data

Intelligent traffic management is widely acknowledged as a means to optimise the utilisation of existing infrastructure capacities. A major requirement for intelligent traffic management is the collection of high quality data on traffic conditions in order to generate accurate real-time traffic information. The approach to be described here generates this information by a fleet of taxis equipped with GPS which act as Floating-Car-Data (FCD) provider for a number of metropolitan areas. The first part of this paper describes the methodology of setting up this data base. The information collected enables various applications such as real-time traffic monitoring, time-dynamic routing and fleet management. The second part of the paper proposes a framework for using these data additionally to include environmental effects into intelligent traffic management systems. To this end, a mapping between travel times and traffic flows is proposed. Some challenges related to the computation of emissions from velocity profiles are discussed. Equipped with these ingredients, an environmentally friendly intelligent traffic management might be in reach

Towards area-wide traffic monitoring-applications derived from probe vehicle data

Comprehensive, up-to-date traffic monitoring is the basis for mobility information and traffic management systems. However, conventional stationary traffic data measurements are hardly able to provide the necessary data for an area-wide monitoring and cannot deliver enough information for many traffic related services. Therefore an al-ternative approach using positioning data of commercial vehicle fleets for traffic monitoring issues has been established. This paper surveys differnt prototype applications based on this probe vehicle data. Continuous monitoring and information of traffic situation via the World Wide Web accomplished by jam detection and highlighting is the basic service. Further on, vehicle route guidance systems using current and historic data achieve superior performance. Such guidance systems have been tested as modules for dynamic navigation and fleet disposition system. Finally a method to derive digital road maps and street characteristics from positioning data is presented

Cloud computing as a new technology trend in education

The construction and operation of extremely large-scale, commodity-computer datacenters was the key necessary enabler of Cloud Computing. Cloud Computing could offer services make a good profit for using in education. With Cloud Computing it is possible to increase the quality of education, improve communicative culture and give to teachers and students new application opportunities

Activation of Rac-1 and RhoA contributes to podocyte injury in chronic kidney disease

Rho-family GTPases like RhoA and Rac-1 are potent regulators of cellular signaling that control gene expression, migration and inflammation. Activation of Rho-GTPases has been linked to podocyte dysfunction, a feature of chronic kidney diseases (CKD). We investigated the effect of Rac-1 and Rho kinase (ROCK) inhibition on progressive renal failure in mice and studied the underlying mechanisms in podocytes. SV129 mice were subjected to 5/6-nephrectomy which resulted in arterial hypertension and albuminuria. Subgroups of animals were treated with the Rac-1 inhibitor EHT1846, the ROCK inhibitor SAR407899 and the ACE inhibitor Ramipril. Only Ramipril reduced hypertension. In contrast, all inhibitors markedly attenuated albumin excretion as well as glomerular and tubulo-interstitial damage. The combination of SAR407899 and Ramipril was more effective in preventing albuminuria than Ramipril alone. To study the involved mechanisms, podocytes were cultured from SV129 mice and exposed to static stretch in the Flexcell device. This activated RhoA and Rac-1 and led via TGFβ to apoptosis and a switch of the cells into a more mesenchymal phenotype, as evident from loss of WT-1 and nephrin and induction of α-SMA and fibronectin expression. Rac-1 and ROCK inhibition as well as blockade of TGFβ dramatically attenuated all these responses. This suggests that Rac-1 and RhoA are mediators of podocyte dysfunction in CKD. Inhibition of Rho-GTPases may be a novel approach for the treatment of CKD

Multiparameter RNA and Codon Optimization: A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expression

Autologous expression of recombinant human proteins in human cells for biomedical research and product development is often hampered by low expression yields limiting subsequent structural and functional analyses. Following RNA and codon optimization, 50 candidate genes representing five classes of human proteins – transcription factors, ribosomal and polymerase subunits, protein kinases, membrane proteins and immunomodulators – all showed reliable, and 86% even elevated expression. Analysis of three representative examples showed no detrimental effect on protein solubility while unaltered functionality was demonstrated for JNK1, JNK3 and CDC2 using optimized constructs. Molecular analysis of a sequence-optimized transgene revealed positive effects at transcriptional, translational, and mRNA stability levels. Since improved expression was consistent in HEK293T, CHO and insect cells, it was not restricted to distinct mammalian cell systems. Additionally, optimized genes represent powerful tools in functional genomics, as demonstrated by the successful rescue of an siRNA-mediated knockdown using a sequence-optimized counterpart. This is the first large-scale study addressing the influence of multiparameter optimization on autologous human protein expression

Highly conducting perylene radical salts

Temperature dependent dc and microwave conductivity data together with EPR and optical reflectance measurements on the "mixed" system (pe)2(ASF6)0,75(PF6)0,35 times 0,85 CH2Cl2 are described. The data prove metallic behaviour of this organic solid down to 200 K

Проектирование автономного быстровозводимого защитного гидротехнического сооружения для местности с периодическими затоплениями

В данной выпускной квалификационной работе был проведен анализ паводковой ситуации за 20 лет. Рассмотрены инженерно-технические и организационные мероприятия по защите населения от наводнения. Исследованы современные типы быстровозводимых водоналивных дамб. Разработан проект быстровозводимого гидротехнического сооружения.In this final qualification work the analysis of a flood situation for 20 years was carried out. Technical and organizational actions for protection of the population against a flood are considered. The modern types of pre-fabricated water filling dams are investigated. The project of the pre-fabricated hydraulic engineering construction is develope